Structure and distribution of organelles and cellular location of calcium transporters in Neurospora crassa

摘要: We wanted to examine the cellular locations of four Neurospora crassa proteins that transport calcium. However, structure and distribution organelles in live hyphae N. have not been comprehensively described. Therefore, we made recombinant genes generate translational fusions putative organellar marker with green or red fluorescent protein. observed endoplasmic reticulum proteins, encoded by grp-78 dpm, nuclear envelope associated membranes. Proteins vacuolar membrane, vam-3 vma-1, were an interconnected network small tubules vesicles near hyphal tip, while more distal regions they large spherical vacuoles. Mitochondria, visualized tagged ARG-4, abundant all hyphae. Similarly, calcium protein their locations. NCA-1 protein, a homolog SERCA-type Ca2+-ATPase animal cells, colocalized markers. The NCA-2 NCA-3 are homologs Ca2+-ATPases membrane yeast plasma cells. They markers also occurred than 1 mm from tip. cax gene encodes Ca2+/H+ exchange found As expected, CAX localized compartment. observed, approximately 50 100 μm few had high amounts subunits ATPase (VMA-1 VMA-5). suggest this organelle, described previously crassa, may role sequestering