Nucleic Acid Capture Assay: A Novel High- Throuhgput Method for Direct Mrna Quantitation

摘要: Generating industrial production cell lines with high productivity is a labor-intensive process limited throughput. As part of this process, hundreds clones are screened using immunoassay and count data in multiple plates dilutions, significantly decreasing the throughput traditional method. To overcome these limitations we developed new solution phase hybridization technique, called Nucleic Acid Capture Assay (NACA) that allows quantification specific mRNA species directly from lysed cells. Target mRNAs captured on solid support signaled through sequence-specific synthetic oligos. Detection capture event achieved probes labeled digoxigenin combined alkaline phosphatase conjugated anti-digoxigenin antibody. In current study, show correlates most well NACA assay can high-throughput clone screening during drug development.