Label-free fluorescent assay of T4 polynucleotide kinase phosphatase activity based on G-quadruplexe-thioflavin T complex.
作者： Hengzhi Zhao , Qiang Liu , Mei Liu , Yan Jin , Baoxin Li
DOI: 10.1016/J.TALANTA.2017.01.027
关键词: Enzyme 、 Phosphatase 、 Thioflavin 、 Biochemistry 、 Oligonucleotide 、 Fluorescence 、 Substrate (chemistry) 、 Chemistry 、 DNA 、 Terminal deoxynucleotidyl transferase
摘要: … tool enzyme with 5′-kinase and 3′-phosphatase activities. Considering its important roles in the repair of strand breaks, assay of T4 PNKP activity is of great importance. In this work, …
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