A label-free assay for T4 polynucleotide kinase/phosphatase activity and its inhibitors based on poly(thymine)-templated copper nanoparticles

作者: Zhen-Zhen Dong , Lin Zhang , Min Qiao , Jia Ge , Ai-Ling Liu

DOI: 10.1016/J.TALANTA.2015.08.055

关键词: ThymineBiochemistryDNA repairDNA polymeraseChemistryDNAPhosphorylationFluorescencePhosphatasePrimer (molecular biology)

摘要: … DNA 3′-phosphatase takes an important role in DNA damage repair, replication and recombination. Here, we present a novel label-free fluorescent assay for T4 polynucleotide kinase/…

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