Transposase A binding sites in the attachment sites of bacteriophage Mu that are essential for the activity of the enhancer and A binding sites that promote transposition towards Fpro-lac

摘要: In this paper we determine which of the A binding sites in attachment phage Mu are required for stimulatory activity transpositional enhancer (IAS). For purpose transposition frequencies mini-Mu's with different truncated to an Ftet target were measured both presence and absence IAS. The results show that our vivo assay L3 R3 dispensable functioning An additional deletion L2 or R2 however abolishes stimulating suggesting interaction between molecules bound these residual a IAS-containing mini (and R3) deleted is much lower than comparable construct without This means IAS inhibiting transposition. Such inhibition not observed when L3) deleted. suggests interacts ordered fashion, first attL then attR. Furthermore mini-Mu enhanced Fpro-lac used as instead Ftet. We elevated dependent on L2,L3 R2. These sequences could possibly mediate plasmid present Fpro-lac.