Th1 and Th2 cytokine responses after measles antigen stimulation in vitro in bone marrow transplant patients: response to measles vaccination

摘要: In seronegative autologous bone marrow transplanted (ABMT) patients, a sustained cell-mediated immunity (CMI) has been shown to impair the antibody response after measles vaccination. To investigate if this might be caused by preferential Th1 cytokine response, interferon (IFN)-gamma and interleukin (IL)-10 production of peripheral blood mononuclear cells (PBMC) was analyzed antigen (M-ag) stimulation in vitro. The non-specific immune measured IFN-alpha, IL-12 analyses. Fifty non-vaccinated patients following ABMT or allogeneic transplantation (BMT) were included. IFN-gamma significantly higher with retained CMI than without (2.3 vs 0.8 IU/ml; P = 0.01). Only non-significant tendency seen IL-10 (48.6 26.7 pg/ml; NS), whereas no difference found IFN-alpha production. A positive correlation between (r(s) 0.49; < 0.001). After vaccination 14 children, there an increase PBMC vitro (2.5 <0.1 0.05), changes IL-10, levels. These results suggest that both Th2 are increased M-ag measles, but seems stronger. may lead reduction humoral which explain negative T cell reactivity prior