A microscopic view of the mechanisms of active transport across the cellular membrane

摘要: Abstract Membrane transporters are molecular machines that couple active transport of their specific substrates to various sources cellular energy through a set highly coordinated protein conformational changes. The alternating-access mechanism in these proteins, which ensures the substrate is only accessible from one side membrane at any given time, relies on complex and global changes also closely coupled events such as binding translocation. In this review, we describe application advanced modeling simulation technologies number proteins studied our laboratory. goal demonstrate power methods describing functionally relevant ranging more localized gating motions large, governing transition between major functional states.