Nucleotide sequence surrounding multiple polyadenylation sites in the mouse dihydrofolate reductase gene.
作者： D R Setzer , M McGrogan , R T Schimke
DOI: 10.1016/S0021-9258(18)34647-7
关键词: Nucleotide 、 Biology 、 Genetics 、 Dihydrofolate reductase 、 Polyadenylation 、 Untranslated region 、 Biochemistry 、 Long non-coding RNA 、 Nucleic acid sequence 、 Sequence (medicine) 、 RNA 、 Cell biology 、 Molecular biology
摘要: We have previously reported the presence of four dihydrofolate reductase messenger RNAs differing in length 3' untranslated regions murine cells (Setzer, D. R., McGrogan, M., Nunberg, J. H., and Schimke, R. T. (1980) Cell 22, 361-370). now mapped ends these more precisely demonstrated colinearity between their shared sequences. Analysis three larger has shown that RNA species contain very long noncoding regions, bringing total number to seven, ranging from 750 5600 nucleotides. determined nucleotide sequence at surrounding polyadenylation sites smaller RNAs. find no striking structures this might constitute multiple signals, but conclude putative signal AAUAAA is not required for least messengers.
elsevier.com
sci-hub.st 参考文章(24)
James C Alwine, David J Kemp, Barbara A Parker, Jakob Reiser, Jaime Renart, George R Stark, Geoffrey M Wahl, None, [15] Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper Methods in Enzymology. ,vol. 68, pp. 220- 242 ,(1979) , 10.1016/0076-6879(79)68017-5
J L Bennetzen, B D Hall, The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase. Journal of Biological Chemistry. ,vol. 257, pp. 3018- 3025 ,(1982) , 10.1016/S0021-9258(19)81067-0
R E Kellems, R T Schimke, J R Bertino, F W Alt, Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells. Journal of Biological Chemistry. ,vol. 253, pp. 1357- 1370 ,(1978) , 10.1016/S0021-9258(17)34875-5
F W Alt, R E Kellems, R T Schimke, Synthesis and degradation of folate reductase in sensitive and methotrexate-resistant lines of S-180 cells. Journal of Biological Chemistry. ,vol. 251, pp. 3063- 3074 ,(1976) , 10.1016/S0021-9258(17)33500-7
R R Fall, M Glaser, P R Vagelos, Acetyl coenzyme A carbosylase. Circular dichroism studies of Escherichia coli biotin carboxyl carrier protein. Journal of Biological Chemistry. ,vol. 251, pp. 2063- 2069 ,(1976) , 10.1016/S0021-9258(17)33655-4
R E Kellems, F W Alt, R T Schimke, Regulation of folate reductase synthesis in sensitive and methotrexate-resistant sarcoma 180 cells. In vitro translation and characterization of folate reductase mRNA. Journal of Biological Chemistry. ,vol. 251, pp. 6987- 6993 ,(1976) , 10.1016/S0021-9258(17)32931-9
Jack H. Nunberg, Randal J. Kaufman, Annie C.Y. Chang, Stanley N. Cohen, Robert T. Schimke, Structure and genomic organization of the mouse dihydrofolate reductase gene. Cell. ,vol. 19, pp. 355- 364 ,(1980) , 10.1016/0092-8674(80)90510-3
R. Heilig, F. Perrin, F. Gannon, J.L. Mandel, P. Chambon, The ovalbumin gene family: structure of the X gene and evolution of duplicated split genes. Cell. ,vol. 20, pp. 625- 637 ,(1980) , 10.1016/0092-8674(80)90309-8
A. M. Maxam, W. Gilbert, A new method for sequencing DNA Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 560- 564 ,(1977) , 10.1073/PNAS.74.2.560
H.C. Birnboim, J. Doly, A rapid alkaline extraction procedure for screening recombinant plasmid DNA Nucleic Acids Research. ,vol. 7, pp. 1513- 1523 ,(1979) , 10.1093/NAR/7.6.1513