Structure and genomic organization of the mouse dihydrofolate reductase gene.
作者： Jack H. Nunberg , Randal J. Kaufman , Annie C.Y. Chang , Stanley N. Cohen , Robert T. Schimke
DOI: 10.1016/0092-8674(80)90510-3
关键词:
摘要: Abstract The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization specific cDNA sequences to restriction endonuclease-generated fragments DNA from methotrexate-resistant S-180 cells. contains a minimum five intervening (one in 5′ untranslated region and four protein-coding region) spans 42 kilobase pairs on genome. Genomic at junction sequence mRNA-coding polyadenylation site have determined. A similar is found independently isolated cell lines, parental sensitive line several inbred strains, indicating that this represents natural gene.
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