Recognition of Initiation Sites in Eukaryotic Messenger RNAs

摘要: The mechanism by which ribosomes select the correct AUG codon for initiation of protein synthesis has been subject considerable speculation and experimentation. In case prokaryotic messages ribosomes, there is convincing evidence supporting Shine Dalgarno’s proposal (1) that base pairing occurs between pyrimidine-rich 3′-end 16S ribosomal RNA a purine-rich sequence located approximately 10 nucleotides to left or GUG initiator (2–4). This interaction plays central role in recognition sites E. coli although additional features message also influence efficiency ribosome binding (5–8). Eukaryotic translational systems display certain peculiarities argue against notion eukaryotes more-or-less an extension accepted mechanism. For example, monocistronic character eukaryotic messenger RNAs (9–11) facilitating effect 5′-terminal m7G cap (12) are features, not observed prokaryotes, must be assimilated into any model eukaryotes.