High-yield production of a human therapeutic protein in tobacco chloroplasts.

摘要: Transgenic plants have become attractive systems for production of human therapeutic proteins because the reduced risk mammalian viral contaminants, ability to do large scale-up at low cost, and maintenance requirements. Here we report a feasibility study protein through transplastomic transformation technology, which has additional advantage increased biological containment by apparent elimination transmission transgenes pollen. We show that chloroplasts can express secretory protein, somatotropin, in soluble, biologically active, disulfide-bonded form. High concentrations recombinant accumulation are observed (>7% total soluble protein), more than 300-fold higher similar gene expressed using nuclear transgenic approach. The plastid-expressed somatotropin is nearly devoid complex post-translational modifications, effectively increasing amount usable protein. also describe approaches obtain with non-methionine N terminus, native results indicate highly efficient vehicle potential pharmaceutical plants.