Expression of Recombinant Proteins via the Plastid Genome

摘要: Genetic engineering of plant cells was first accomplished more than 20 years ago. Since this time, nuclear transgenic plants have been used for everything from the study gene function to use as production vehicles industrial enzymes. However, contain two additional genetic compartments—plastids and mitochondria—for which technology could provide exciting alternative approaches over existing methods transformation. Although mitochondrial transformation has not yet achieved, ability incorporate transgenes interest directly into plastid genome higher via became a reality in early 1990s (1,2), following similar success unicellular green alga Chlamydomonas reinhardtii (3). Compared transformation, several potential advantages protein may exist (Table 1). These include accumulate extraordinarily high levels recombinant proteins, some cases up 30–40% total cell. In contrast random integration observed transgene is exclusively directed by homologous recombination. Therefore, all events derived single vector predictable insert quality uniform gene-expression characteristics. Also, silencing plastids, so subsequent generations maintain faithful expression genes interest.