Relocation of the plastid rbcL gene to the nucleus yields functional ribulose-1,5-bisphosphate carboxylase in tobacco chloroplasts.

摘要: Abstract The conserved plastid localization of rbcL suggests that biosynthesis the large subunit ribulose-1,5-bisphosphate carboxylase [Rubisco; 3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39] in chloroplasts is required to obtain functional enzyme. To examine validity this hypothesis, we relocated gene nucleus. First, deleted from tobacco genome by targeted insertion a selectable aadA encoding spectinomycin resistance. The coding region was then inserted into an expression cassette and introduced nuclear these plants Agrobacterium-mediated transformation. We report functionally complements defective plastids when Rubisco transit peptide. Therefore, evolutionary process relocates genes nucleus has not yet occurred case gene. Targeted deletion genes, combined with their allotopic expression, will provide opportunities for studying function enzyme complexes.