Purification and analysis of RNA from paraffin-embedded tissues.

摘要: We have developed methods for the purification and analysis of RNA from formalin-fixed paraffin-embedded tissue. The allow retrospective gene expression or viral infection. extraction uses prolonged solubilization tissue with detergent protease in presence high concentrations a ribonuclease inhibitor. purified is moderately degraded but its hybridization behavior essentially unaffected. were able to quantify specific mRNAs by dot-blot hybridization.