Isolation of nonsense suppressor mutants in Pseudomonas.

摘要: A strain of Escherichia coli harboring the drug resistance plasmid RP1 was treated with mutagen N-methyl-N-nitro-N-nitro-N-nitrosoguanidine, and mutants were isolated in which ampicillin had been lost due to an amber mutation plasmid. One these again treated, a tetracycline also The containing mutations genes amp tet named pLM2. This could be transferred strains Pseudomonas aeruginosa, P. phaseolicola, pseudoalcaligenes. Mutants resistant not obtained from phaseolicola carrying However, E. coli, pseudoalcaligenes did produce simultaneously both antibiotics. All developed nonsense suppressors since they became phenotypically lac+, although lac mutation, formed plaques phages PRR1 PRD1 that attack organisms RP1. Approximately 20% aeruginosa sensitive mutant PRD1. These variable stability grew somewhat more slowly than their parent strains. suppressor pseudoalcaligenes, designated ERA(pLM2)S4, used for isolation bacteriophage PHA6, virus having segmented genome double-stranded ribonucleic acid envelope lipids proteins.