Essential fatty acid deficiency prevents multiple low-dose streptozotocin-induced diabetes in CD-1 mice.

摘要: Multiple i.p. injections of low-dose streptozotocin (40 mg/kg) produce insulitis, beta cell destruction, and diabetes in male CD-1 mice. Recent data also suggest that macrophages figure the model. Because other recent studies have shown essential fatty acid deficiency prevents autoimmune nephritis mice, decreases number resident Ia-positive glomerular macrophages, elicitation into glomerulus inflammation, we examined effect on incidence severity insulitis mice treated with streptozotocin. Streptozotocin-treated control diet uniformly developed (19/19). Essential acid-deficient did not develop (1/13). Mean plasma glucose levels for were 384.5 +/- 23.6 129.1 15.5 mg/dl, respectively, at end 1 month. To discern whether prevented streptozotocin-induced injury or inflammatory response to injured cells, repleted daily 99% pure methyl linoleate beginning 3 days after last injection. These quickly severe (3/4) mild (1/4) diabetes. Histologic examination pancreata showed marked destruction; contrast, preservation cells only focal peri-insulitis. thus resultant streptozotocin-treated suggesting a central role lipid mediators this autoimmunity