Determination of the order of mutational sites governing l-arabinose utilization in Escherichia coliBr by transduction with phage P1bt☆☆☆
作者： J. Gross , E. Englesberg
DOI: 10.1016/0042-6822(59)90125-4
关键词: Escherichia 、 Biochemistry 、 Biology 、 Group A 、 Transduction (genetics) 、 Ribulose 、 Complementation 、 Sugar 、 Mutant 、 Arabinose
摘要: The order of seventeen l-arabinose mutants Escherichia coliBr has been determined by three- and four-factor transduction experiments with phage P1bt. assortment the markers thr leu, located on either side arabinose cluster, was observed among recombinants between pairs mutants. sites are arranged in a linear sequence. divided into three groups biochemical analysis application abortive test for complementation. Group A (four mutants) accumulates trace amounts keto sugar is inhibited l-arabinose; group B (eight large quantities sugar, ribulose, C (five small (more than A) resistant to l-arabinose. distinguished from tests. Comparison mutant grouping functional criteria demonstrates complete correspondence which they belong. involving were characterized presence marked negative interference.
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