Immunoregulation in Experimental Schistosomiasis: in Vitro Induction and Assay of Spleen Cell Suppressor Activity

摘要: Spleen cells from normal CBA/J mice or infected with Schistosoma mansoni were exposed for 48 to 72 hr either concanavalin A (Con A), soluble egg antigen (SEA), worm antigenic preparation (SWAP), treated mitomycin C prevent further DNA synthesis, and admixed sensitized syngeneic spleen a concentration gradient of phytohemagglutinin (PHA) SEA, respectively. Both nonspecific (by Con A) “antigen-specific” SEA SWAP in only) induction suppression was observed when using PHA-induced blastogenesis as the final assay. The number inducible splenic suppressive activity degree PHA induced by exposure appeared decline between 8 20 weeks infection. In contrast, response served assay, strong all chronically (20 infection). This model permits parallel analysis suppressor schistosome antigen-specific signals during course this chronic, immunoregulated condition, schistosomiasis mansoni.