Suppression of mitogen-induced lymphocyte transformation by plasma from patients with hepatosplenic schistosomiasis mansoni: role of immune complexes.

摘要: Plasma from chronic or advanced hepatosplenic schistosomiasis (mansoni) patients (PCS) suppressed mitogen-induced responses of normal human peripheral blood mononuclear cells. 1 x 10(5) Ficoll-Hypaque isolated lymphocytes were cultured in 100 microliter RPMI 1640 media with plasma preparation and stimulated phytohemagglutinin P (PHA), concanavalin A (Con A), pokeweed mitogen (PWM) Staphylococcus aureus phage lysate (SPL). Compared to plasma, dose-response experiments 10-50% PCS uniformly inhibited the 72-h PHA, Con PWM maximal suppression at 50% concentration. At this concentration, range was 42-60%. The inhibitory activity unaffected by 56 degrees C, 30 min pretreatment PCS. However, pre-culture cells PHA for 24 h prior addition abrogated suppressor activity. Furthermore, 6-day cultures SPL not nearly eliminated pre-treatment 4% (w/v) polyethylene glycol (PEG) all cases. In putative immune complexes PEG precipitates, Clq, C3, C4, IgG, IgM IgA identified samples. Schistosomal antigen found only two 23 samples tested significant anti-schistosomal IgG antibodies precipitates. data suggest (non-specific specific) act suppress mitogenic responses.