Fluorescent measurement of microalgal neutral lipids.

作者: Danielle Elsey , David Jameson , Barry Raleigh , Michael J. Cooney

DOI: 10.1016/J.MIMET.2006.11.008

关键词: FluorescenceNeutral lipidOrganic chemistryNile redHigh-throughput screeningPolarity (physics)ChemistryPhotochemistry

摘要: Nile Red, a dye that fluoresces at defined wavelengths depending upon the polarity of surrounding medium, has been proposed to determine neutral lipid content microalgal cells. Herein we communicate modifications this technique facilitate its use as high-throughput screening technology, well improving accuracy and versatility.

参考文章(19)
Seog June Lee, Byung-Dae Yoon, Hee-Mock Oh, Rapid method for the determination of lipid from the green alga Botryococcus braunii Biotechnology Techniques. ,vol. 12, pp. 553- 556 ,(1998) , 10.1023/A:1008811716448
Craig Laughton, Measurement of the specific lipid content of attached cells in microtiter cultures Analytical Biochemistry. ,vol. 156, pp. 307- 314 ,(1986) , 10.1016/0003-2697(86)90258-7
Yu Suen, J. S. Hubbard, G. Holzer, T. G. Tornabene, Total lipid production of the green alga nannochloropsis sp qii under different nitrogen regimes Journal of Phycology. ,vol. 23, pp. 289- 296 ,(1987) , 10.1111/J.1529-8817.1987.TB04137.X
El Hassan Belarbi, Emilio Molina, Yusuf Chisti, A process for high yield and scaleable recovery of high purity eicosapentaenoic acid esters from microalgae and fish oil. Enzyme and Microbial Technology. ,vol. 26, pp. 516- 529 ,(2000) , 10.1016/S0141-0229(99)00191-X
Yordan Kostov, Peter Harms, Lisa Randers-Eichhorn, Govind Rao, Low-cost microbioreactor for high-throughput bioprocessing. Biotechnology and Bioengineering. ,vol. 72, pp. 346- 352 ,(2001) , 10.1002/1097-0290(20010205)72:3<346::AID-BIT12>3.0.CO;2-X
Keith E. Cooksey, James B. Guckert, Scott A. Williams, Patrik R. Callis, Fluorometric determination of the neutral lipid content of microalgal cells using Nile Red Journal of Microbiological Methods. ,vol. 6, pp. 333- 345 ,(1987) , 10.1016/0167-7012(87)90019-4
Ana P. Carvalho, F. Xavier Malcata, Preparation of fatty acid methyl esters for gas-chromatographic analysis of marine lipids: insight studies. Journal of Agricultural and Food Chemistry. ,vol. 53, pp. 5049- 5059 ,(2005) , 10.1021/JF048788I
Tom Lewis, Peter D Nichols, Tom A McMeekin, Evaluation of extraction methods for recovery of fatty acids from lipid-producing microheterotrophs Journal of Microbiological Methods. ,vol. 43, pp. 107- 116 ,(2000) , 10.1016/S0167-7012(00)00217-7