Optimization of growth conditions toward two-stage cultivation for lipid production of chlorella vulgaris

摘要: Increasing concerns about limited fossil fuel and elevated level of atmospheric carbon dioxide are driving force to develop renewable biofuels, microalgae considered a particularly promising feedstock for industrialized biofuel due their inherent advantages. It is imperative further optimize the growth conditions in order achieve maximal algal biomass productivity low down cost microalgal production. The research this project aimed enhance lipid production Chlorella vulgaris by optimizing nutrient supply conditions. effects temperature, nitrogen concentration initial cell density on neutral were evaluated optimized response surface method with 17-run Box–Behnken design (BBD). With increase temperature from 20 30°C, increased up its peak value at 27°C. However, 2D contour lines showed has significant effect compared those and/or concentration. optimal 1.5 g L−1 50% 27.4°C. obtained experiment was agreement model prediction, confirming validity model. © 2015 American Institute Chemical Engineers Environ Prog, 34: 1801–1807,