Description of Bursaphelenchus populi sp. n. (Nematoda: Parasitaphelenchidae), a new member of the xylophilus group from aspen, Populus tremula L., in Europe.

摘要: Bursaphelenchus populi sp. n. is described from dying and dead aspen trees, Populus tremula , in Poland. The nematode was found trees infested with a long-horn beetle, Saperda perforata vectored the insect haemocoel. characteristic morphology of male spicules, extended anterior vulval lip, lateral fields four incisures, number arrangement caudal papillae, place B. xylophilus group. can be separated all other species that group by distinctive flap, which always bent its distal half sunken conspicuous, sharp depression posterior to vulva, morphological morphometric characters, i.e. female tail shape, excretory pore position, spicule length (32.1 (25.7-37.0) μ m (as measured along arc) relatively long ( L,= 1020 (909-1111) females), L = 850 (756-1055) males), slender body (a 45.4 (40.2-52.4) 42.0 (36.1-49.3) male, respectively). status new confirmed unique molecular profile ITS region (ITS-RFLP). DNA sequencing phylogenetic analysis 28S rDNA placed close fraudulentus In vitro cross-breeding mucronatus doui revealed full reproductive incompatibility between these species. laboratory experiments on 2-year-old seedlings specific aspen. It did not develop pine or oak reproduced only weakened seedlings. No pathogenicity tree host vector observed. Botrytis cinerea PDA cultures, dimensions were significantly altered both sexes differed when compared individuals extracted wood.