Characterization of a Porous Nano-electrospray Capillary Emitter at Ultra-low Flow Rates.

摘要: Biopharmaceuticals, especially therapeutic monoclonal antibodies, have emerged as a very promising new generation of protein-based drugs. However, their comprehensive analysis continues to pose challenges for the bioanalytical field. Hyphenation capillary electrophoresis with electrospray ionization (CE-MS) is technique address these challenges. One main advantages CE-MS ability produce stable at ultra-low flow rates (5-20 nl/min range). In this short communication we report on characterization porous nano-electrospray emitter focusing effects rate efficiency, ion suppression and detection sensitivity. Ion effect poorly-ionizable sugar in presence an easily-ionized peptide was reduced by almost 2-fold. Intact antibody infusion demonstrated that MS sensitivity increased order magnitude decrease from 250 nL/min 20 using emitter.