Content of 8-oxodG in chromosomal DNA of Sparus aurata fish as biomarker of oxidative stress and environmental pollution.

摘要: Abstract The 8-oxodG content has been measured in chromosomal DNA of gilthead seabream ( Sparus aurata ) by HPLC–EC. Susceptibility different tissues to oxidative damage was studied exposing fish model pollutants. Cu(II), paraquat (PQ) and malathion failed promote oxidation liver, while dieldrin significantly increased the this organ, but not gills or blood. After PQ exposure, liver showed high levels glucose-6-P dehydrogenase (G-6PDH) GSSG reductase activities. antioxidant status lack a specific transport system could explain susceptibility induced PQ. Increased were detected PQ-exposed after 8 24 h. In contrast, 48 h exposed contained lower than controls. existence O 2 -rich organ significant increase defenses would sensitivity promoted Elimination soluble chemical putative induction DNA-repair enzymes for damages drop at longer times. Fish moderate urban industrial pollution hepatic DNA. We conclude that determination HPLC–EC is potentially useful biomarker environmental pollution, although its response still somewhat other well-established biomarkers stress.