Magnetic Solid Phase Extraction Coupled with HPLC Towards Removal of Pigments and Impurities from Leaf-derived Paclitaxel Extractions of Taxus baccata and Optimization via Response Surface Methodology

摘要: A reusable and cost-effective magnetic graphite oxide (Fe3O4NPs@GO) nanocomposite was fabricated applied for pre-purification of paclitaxel from leaf-derived crude extract Taxus baccata. Furthermore, the potential roles three crucial criteria (i.e., adsorbent dosage, sorption temperature agitation/shaking power) on two responses [i.e., efficiency plant pigments removal (EPPR) taxol purity (ETP)] were examined simultaneously optimized through response surface methodology. The accurately characterized using TEM, AFM, BET, FT-IR, Raman VSM. Moreover, both proposed second-degree polynomial regression models, highly significant correlations achieved between experimental predicted data (p < 0.0001). Meanwhile, optimum conditions to acquire maximum EPPR (94.0 %) ETP (11.4 recorded as dosage 37.7 g L−1, 30.7 °C agitation power 153.1 rpm; predictive results confirmed rechecking survey. Interestingly, upon five consecutive treatments, still exhibited substantial potency in eliminating large amounts impurities (up 90 %), without reduction capacity magnetism thereof. Our demonstrated that current nanocomposite, SPE sorbent MSPE, could be a simple, fast approach HPLC-based purification studies paclitaxel, probably other secondary metabolites.