Replication and Transmission of H9N2 Influenza Viruses in Ferrets: Evaluation of Pandemic Potential
作者： Hongquan Wan , Erin M Sorrell , Haichen Song , Md Jaber Hossain , Gloria Ramirez-Nieto
DOI: 10.1371/JOURNAL.PONE.0002923
关键词: Virology 、 Influenza A virus subtype H5N1 、 Virus 、 Biology 、 H5N1 genetic structure 、 Microbiology 、 Transmission (medicine) 、 Hemagglutinin (influenza) 、 Viral replication 、 Titer 、 Gene
摘要: H9N2 avian influenza A viruses are endemic in poultry of many Eurasian countries and have caused repeated human infections Asia since 1998. To evaluate the potential threat to humans, we investigated replication transmission efficiency ferret model. Five wild-type (WT) viruses, isolated from different species 1988 through 2003, were tested vivo found replicate ferrets. However these achieved mild peak viral titers nasal washes when compared those observed with a H3N2 virus. Two transmitted direct contact ferrets, however no aerosol was detected virus displaying most efficient transmission. leucine (Leu) residue at amino acid position 226 hemagglutinin (HA) receptor-binding site (RBS), responsible for virus-like receptor specificity, be important In addition, an avian-human reassortant virus, which contains surface glycoprotein genes six internal showed enhanced contacts. Although observed, replicated multiple respiratory tissues induced clinical signs similar parental Our results suggest that establishment prevalence pose significant humans.
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