Roles of carbonic anhydrases in the alimentary tract.

摘要: Insight into the physiological role of carbonic anhydrases (CAs) can be derived from a knowledge cell types expressing enzyme and by correlating activity with particular function at cellular or subcellular site its presence. Several approaches have been used to determine location CA tissue intracellular levels. The specific visualized trapping reaction product heavy metal. Hansson’s histochemical method, depending on cobalt CO2 produced CA, has served identify number sites (Korhonen et al., 1966; Hansson, 1967; O’Brien 1977; Parkkila 1989; Ridderstrale, 1991). availability antibodies different isoenzymes, development modern immunocytochemical techniques developments in molecular biology also helped considerably increase our understanding distribution isoenzymes alimentary tract. Studies effects inhibitors implicated CAs variety processes, although several problems encountered these due their unspecific mode action. In addition, human animal deficiencies may provide substantial improvements individual contributions each isoenzyme processes.