Parvalbumin expression in the claustrum of the adult dog. An immunohistochemical and topographical study with comparative notes on the structure of the nucleus.

摘要: Although the detailed structure and function of claustrum remain enigmatic, its extensive reciprocal connection with cortex suggests a role in integration multisensory information. Claustrum samples, obtained from necropsy four dogs, were formalin fixed for paraffin embedding. Sections either stained morpho-histological analysis or immunostained parvalbumin (PV). We focused on PV because cortical hippocampal areas it is marker fast-spiking interneurons which have an important information transmission processing. Soma area, perimeter circularity considered as morphological parameters to quantitatively group positive somata by k-means clustering. The histological investigation revealed superior pyramidoid puddle posterior characterized "cloud" neurons dorso-lateral part. Immunostaining showed fibers throughout rostro-caudal extent dog claustrum, localized principally dorsal region. k-Means clustering enabled neuron classification according size, identifying respectively big (radius=11.42±1.99μm) small (radius=6.33±1.08μm) cells. No statistical differences soma shape observed. topographical distribution immunoreactivity that might be functionally related processing visual inputs. Taken together our findings may help understanding physiology when compared anatomical functional data other species.