Efeitos da radiação gama no comportamento mecânico e na calcificação do pericárdio bovino fixado com glutaraldeído
作者： José Augusto Baucia , Ricardo Mendes Leal Neto , José Roberto Rogero , Nanci do Nascimento , Carmem Aparecida Cruz Reyes
DOI: 10.1590/S0102-76382007000100014
关键词: Bovine pericardium 、 Nuclear medicine 、 Gamma irradiation 、 Medicine
摘要: AbstractObjective: To evaluate the effect of gamma irradiation onglutaraldehyde-fixed bovine pericardium. Method: Glutaraldehyde-fixed pericardium wasexposed to radiation (doses from 0 10000 Gy). Sixsamples each nine groups were evaluated by opticmicroscopy, and shrinking mechanical tests thedenaturation temperature was determined. Additionally, theywere subcutaneously implanted in rats after four monthsthey explanted Ca 2+ levels measured atomicabsorption spectroscopy. Results: The (in µg/mg): control (0 Gy) -194.45; 50 Gy - 154.64; 100 169.37; 200 163.64; 500Gy 199.89; 1000 184.02; 2000 198.95; 5000 -227.95; 362.62. Gamma caused asignificant on biomechanical properties tissue. Conclusion: Exposure did not reduceCa a significant reduction tensilestrength glutaraldehyde-fixed Descriptors:
ipen.br 
sci-hub.st 参考文章(21)
R A Grant, C M Kent, R W Cox, The effects of gamma irradiation on the structure and reactivity of native and cross-linked collagen fibres. Journal of Anatomy. ,vol. 115, pp. 29- 43 ,(1973)
Vinicius José da Silva Nina, Pablo Maria Alberto Pomerantzeff, Ivan Sérgio Joviano Casagrande, David Chung, Carlos Manuel de Almeida Brandão, Sérgio Antonio Barbosa do Nascimento, Luiz Alberto Benvenuti, Sérgio Almeida de Oliveira, In vivo endothelialization of cardiac bioprostheses: conventional versus non-aldehyde preservation Brazilian Journal of Cardiovascular Surgery. ,vol. 19, pp. 144- 151 ,(2004) , 10.1590/S0102-76382004000200008
George Ronald Soncini da Rosa, Francisco Diniz Affonso da Costa, Ricardo Guilherme D'Otaviano de Cas Vilani, Felipe Phol de Souza, Lorena Reichert, Iseu de Santo Elias Affonso da Costa, Utilização do etanol na prevenção da calcificação em heteroenxerto valvar pulmonar porcino: estudo experimental em ovinos Brazilian Journal of Cardiovascular Surgery. ,vol. 21, pp. 304- 313 ,(2006) , 10.1590/S0102-76382006000300010
Y.M. Hafeez, A.B.Z. Zuki, N. Yusof, H. Asnah, M.Y. Loqman, M.M. Noordin, M.Y. Ainul-Yuzairi, Effect of Freeze Drying and Gamma Irradiation on Biomechanical Properties of Bovine Pericardium Cell and Tissue Banking. ,vol. 6, pp. 85- 89 ,(2005) , 10.1007/S10561-004-1888-Z
F.J. Schoen, R.J. Levy, Bioprosthetic heart valve calcification: membrane-mediated events and alkaline phosphatase Bone and Mineral. ,vol. 17, pp. 129- 133 ,(1992) , 10.1016/0169-6009(92)90723-Q
Danielle Hirsch, Frederick J. Schoen, Robert J. Levy, Effects of metallic ions and diphosphonates on inhibition of pericardial bioprosthetic tissue calcification and associated alkaline phosphatase activity Biomaterials. ,vol. 14, pp. 371- 377 ,(1993) , 10.1016/0142-9612(93)90057-9
Yashwant V Pathak, James Boyd, Robert J Levy, Frederick J Schoen, Prevention of calcification of glutaraldehyde pretreated bovine pericardium through controlled release polymeric implants: studies of Fe3+, Al3+, protamine sulphate and levamisole. Biomaterials. ,vol. 11, pp. 718- 723 ,(1990) , 10.1016/0142-9612(90)90034-N
Thomas P. Johnston, Frederick J. Schoen, Robert J. Levy, Prevention of Calcification of Bioprosthetic heart Valve Leaflets by Ca2+ Diphosphonate Pretreatment Journal of Pharmaceutical Sciences. ,vol. 77, pp. 740- 744 ,(1988) , 10.1002/JPS.2600770903
P. M. KOPP, J. F. READ, A. CHARLESBY, COMPARISON OF THE EFFECT OF ALPHA AND GAMMA RADIATION ON AN ENZYME Nature. ,vol. 211, pp. 959- 960 ,(1966) , 10.1038/211959B0
Nanci Do Nascimento, Corrine S. Seebart, Brian Francis, José Roberto Rogero, Ivan I. Kaiser, Influence of ionizing radiation on crotoxin : biochemical and immunological aspects Toxicon. ,vol. 34, pp. 123- 131 ,(1996) , 10.1016/0041-0101(95)00111-5