Development of Rapid Isothermal Amplification Assays for Detection of Phytophthora spp. in Plant Tissue.

摘要: ABSTRACT Several isothermal amplification techniques recently have been developed that are tolerant of inhibitors present in many plant extracts, which can reduce the need for obtaining purified DNA running diagnostic assays. One such commercially available technique has similarities with real-time polymerase chain reaction (PCR) designing primers and a labeled probe is recombinase (RPA). This technology was used to develop two simple rapid approaches detection Phytophthora spp.: one genus-specific assay multiplexed internal control other species-specific assays ramorum P. kernoviae. All were tested sensitivity (ranging from 3 ng 1 fg DNA) specificity using extracted more than 136 taxa, 21 Pythium spp., Phytopythium sp., wide range species. The lower limit linear 200 300 all pathogen RPA Six differ...