Characterization of Phytophthora hybrids from ITS clade 6 associated with riparian ecosystems in South Africa and Australia.
作者： Jan H. Nagel , Marieka Gryzenhout , Bernard Slippers , Michael J. Wingfield , Giles E.St.J. Hardy
DOI: 10.1016/J.FUNBIO.2013.03.004
关键词: Phytophthora 、 Phylogenetic tree 、 Clade 、 Internal transcribed spacer 、 Genetics 、 Allele 、 Biology 、 Phylogenetics 、 Locus (genetics) 、 Hybrid
摘要: Surveys of Australian and South African rivers revealed numerous Phytophthora isolates residing in clade 6 the genus, with internal transcribed spacer (ITS) gene regions that were either highly polymorphic or unsequenceable. These suspected to be hybrids. Three nuclear loci, ITS region, two single copy loci (antisilencing factor (ASF) G protein alpha subunit (GPA)), one mitochondrial locus (cytochrome oxidase c I (coxI)) amplified sequenced test this hypothesis. Abundant recombination within region was observed. This, combined phylogenetic comparisons other three confirmed presence four different hybrid types involving described parent species amnicola, thermophila, taxon PgChlamydo. In all cases, only a coxI allele detected, suggesting hybrids arose from sexual recombination. All sterile culture their physiological traits tended resemble those maternal parents. Nothing is known regarding host range pathogenicity. Nonetheless, as several Western Australia obtained rhizosphere soil dying plants, they should regarded potential threats plant health. The frequent occurrence strongly suggests an origin subsequent introduction into Africa.
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