Aneuploidy in pluripotent stem cells and implications for cancerous transformation

摘要: Owing to a unique set of attributes, human pluripotent stem cells (hPSCs) have emerged as promising cell source for regenerative medicine, disease modeling and drug discovery. Assurance genetic stability over long term maintenance hPSCs is pivotal in this endeavor, but can adapt life culture by acquiring non-random changes that render them more robust easier grow. In separate studies between 12.5% 34% hPSC lines were found acquire chromosome abnormalities time, with the incidence increasing passage number. The predominant involve number structure (particularly chromosomes 1, 12, 17 20), reminiscent observed cancer cells. review, we summarize current knowledge on causes consequences aneuploidy highlight potential links cancers early embryos. We point need comprehensive characterization mechanisms underpinning both acquisition chromosomal selection pressures, which allow mutations persist cultures. Elucidation these will help design conditions minimize appearance aneuploid hPSCs. Moreover, may provide platform analyse driving forces behind genome evolution eventually lead cancerous transformation.