Nanofiber scaffolds with gradients in mineral content for spatial control of osteogenesis.

摘要: Reattachment of tendon to bone has been a challenge in orthopedic surgery. The disparate mechanical properties the two tissues make it difficult achieve direct surgical repair tendon-to-bone insertion. Healing after typically does not regenerate natural attachment, complex tissue that connects and across gradient both mineral content cell phenotypes. To facilitate regeneration our groups have developed nanofiber-based scaffold with graded coating mimic composition native In present work, we evaluated ability this induce osteogenesis adipose-derived mesenchymal stem cells (ASCs). Results from 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay proliferating nuclear antigen staining indicated proliferation was negatively correlated content. contrast, alkaline phosphatase staining, an indicator osteogenesis, positively Likewise, runt-related transcription factor 2 (an early marker osteoblast differentiation) osteocalcin (a late immunostaining were These results indicate on surface nanofiber is capable inducing differentiation ASCs into osteoblasts for enthesis repair.