Tetrazine-based bio-orthogonal coupling reagents and methods
作者： Melissa Blackman , Joseph Michael Fox , Maksim Royzen , Ni Yan
DOI:
关键词: Aqueous medium 、 Coupling reaction 、 Coupling (electronics) 、 Covalent bond 、 Chemistry 、 Primary (chemistry) 、 Reagent 、 Tetrazine 、 Cyclopropene 、 Organic chemistry
摘要: Coupling reactions, suitable for use in organic or aqueous media, are performed by contacting a 1,2,4,5-tetrazine with dienophile. The dienophile may be covalently bonded to protein, and the coupling reaction biological media such as those containing cells cell lysates. reactions presence of primary amines, thiols, acetylenes, azides, phosphines, products Staudinger and/or Sharpless-Huisgen Novel 3-substituted cyclopropene compounds trans-cyclooctenes exemplary dienophiles these reactions.
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