DNA replication and late protein synthesis during SP82 infection of Bacillus subtilis

摘要: Abstract During infection of Bacillus subtilis by bacteriophage SP82, substantial quantities at least two different late proteins were synthesized, even in the absence detectable phage DNA replication. The lytic enzyme (which is possibly a host specific enzyme) and protein(s) responsible for serum blocking power presumably specific). replication was assured use an SP82 double mutant carrying mutation each cistrons required replication, verified density transfer isotope incorporation experiments. Thus, it appears that not protein synthesis during infection. Using closely related phage, 2C, Pene Marmur (1967) had shown that, when inhibited mitomycin C, early synthesized normally, but not. We have repeated this observation with also prevents appearance power, eliminating possibility effect to proteins. However, we suitable preincubation, can inhibit synthesis. suggest differential on merely result time after application which groups are normally synthesized. Gage Geiduschek (1971) showed defective SP01, another failed synthesize one class RNA. discuss possible ways our data be reconciled result.