Protocol for the Molecular Detection of Antibiotic Resistance Mechanisms in Neisseria gonorrhoeae

摘要: Gonorrhoea is no longer an easily treatable ailment but rather now a challenging disease in terms of antimicrobial resistance (AMR) with treatment options rapidly diminishing. The causative agent gonorrhoea, Neisseria gonorrhoeae , has managed to develop almost every single drug used against it the sole exception extended spectrum cephalosporins. situation further exacerbated by fact that not only are rates gonococcal infections on steady rise globally, tracking AMR being undermined growing popularity molecular methods at expense traditional bacterial culture diagnostic laboratories. Recently, concerns have been raised over emergence multi-resistant gonococci and potential for untreatable gonorrhoea. Maintaining optimal epidemiological surveillance remains important aspect gonorrhoea control. development tools N. enhance such surveillance. In this chapter, we discuss nucleic acid ampli fi cation-based detection particular emphasis chromosomal-mediated beta-lactam antibiotics.