A cholera toxin-sensitive G-protein stimulates exocytosis in sea urchin eggs.
作者： Paul R. Turner , Laurinda A. Jaffe , Paul Primakoff
DOI: 10.1016/0012-1606(87)90260-0
关键词: Exocytosis 、 Cholera toxin 、 EGTA 、 Biology 、 G protein 、 Biochemistry 、 Lytechinus variegatus 、 Molecular biology 、 Sea urchin 、 Cortical reaction 、 Pertussis toxin
摘要: To identify guanine nucleotide binding proteins (G-proteins) in sea urchin eggs and to investigate their role signal transduction at fertilization, we used cholera toxin (CTX) pertussis (PTX), which catalyze the specific ADP-ribosylation of G-proteins. Cell surface complex, consisting plasma membranes adhering cortical vesicles, was prepared from Lytechinus variegatus incubated with 32P-labeled NAD presence CTX or PTX. catalyzed a 47-kDa polypeptide, whereas PTX 40-kDa polypeptide. Microinjection approximately 30 micrograms/ml whole 20 subunit A into intact caused exocytosis vesicles. However, if were first injected EGTA (0.6-1.4 mM), injection did not cause exocytosis. Eggs 0.8-2.8 mM cAMP 1.0-4.0 adenosine 3':5'-monophosphotioate cyclic Sp-isomer (cAMP-S), hydrolysis-resistant analog cAMP, undergo These results suggest that CTX-sensitive G-protein is involved regulating Ca2+ release vesicles eggs.
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