Purification and properties of the egg plasma membrane.

摘要: Publisher Summary The chapter focuses on the preparation of plasma membranes from unfertilized and fertilized eggs that are low in contamination by other organelles that, vesicle form, amenable to biochemical analysis. describes details these methods presents some ways membrane preparations can be used. is greatly facilitated fact large sheets with associated cortical vesicles (cell surface complex) isolated quickly good yield. then released sheets, which purified differential density gradient centrifugation. This method yields retain many vitelline layer components including sperm receptors. egg morphology, enzymatic activity membrane, protein composition characterized. presented allow useful quantities sea urchin egg. These exhibit enzyme activities at least transport properties characteristic eukaryotic membranes. routinely used study fertilization-dependent changes tyrosine kinase they should for enzymes as well.