Isolation and characterization of MOD5, a gene required for isopentenylation of cytoplasmic and mitochondrial tRNAs of Saccharomyces cerevisiae.
作者： M E Dihanich , D Najarian , R Clark , E C Gillman , N C Martin
DOI: 10.1128/MCB.7.1.177
关键词: Transferase 、 Biology 、 Mutant 、 Transfer RNA 、 Saccharomyces cerevisiae 、 Structural gene 、 Mitochondrion 、 Biochemistry 、 TRNA modification 、 TRNA isopentenyltransferase 、 Molecular biology
摘要: The mod5-1 mutation is a nuclear in Saccharomyces cerevisiae that reduces the biosynthesis of N6-(delta 2-isopentenyl)adenosine both cytoplasmic and mitochondrial tRNAs to less than 1.5% wild-type levels. tRNA modification enzyme, delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase, cannot be detected vitro with extracts from cells. A characterization MOD5 gene would help determine how same enzyme activity different cellular compartments can abolished by single mutation. To end we have cloned shown it restores transferase mitochondria nucleus/cytoplasm yeast That sequences are expressed Escherichia coli complement an 2-isopentenyl)-2-methylthioadenosine-deficient E. mutant leads us conclude structural for transferase.
sci-hub.se 参考文章(39)
Ronald W. Davis, Marjorie Thomas, John Cameron, Thomas P. St. John, Stewart Scherer, Richard A. Padgett, [49] Rapid DNA isolations for enzymatic and hybridization analysis Nucleic Acids Part I. ,vol. 65, pp. 404- 411 ,(1980) , 10.1016/S0076-6879(80)65051-4
P. Senapathy, M.T. Jacob, Identification and purification of tRNAs containing N6-(delta 2-isopentenyl) adenosine using antibodies specific for N6-(delta-isopentenyl) adenosine. Journal of Biological Chemistry. ,vol. 256, pp. 11580- 11584 ,(1981) , 10.1016/S0021-9258(19)68441-3
S R Ellis, M J Morales, J M Li, A K Hopper, N C Martin, Isolation and characterization of the TRM1 locus, a gene essential for the N2,N2-dimethylguanosine modification of both mitochondrial and cytoplasmic tRNA in Saccharomyces cerevisiae. Journal of Biological Chemistry. ,vol. 261, pp. 9703- 9709 ,(1986) , 10.1016/S0021-9258(18)67571-4
N C Martin, A K Hopper, Isopentenylation of both cytoplasmic and mitochondrial tRNA is affected by a single nuclear mutation. Journal of Biological Chemistry. ,vol. 257, pp. 10562- 10565 ,(1982) , 10.1016/S0021-9258(18)33857-2
A.P. van Loon, E.T. Young, Intracellular sorting of alcohol dehydrogenase isoenzymes in yeast: a cytosolic location reflects absence of an amino-terminal targeting sequence for the mitochondrion. The EMBO Journal. ,vol. 5, pp. 161- 165 ,(1986) , 10.1002/J.1460-2075.1986.TB04191.X
J P Beltzer, L F Chang, A E Hinkkanen, G B Kohlhaw, Structure of yeast LEU4. The 5' flanking region contains features that predict two modes of control and two productive translation starts. Journal of Biological Chemistry. ,vol. 261, pp. 5160- 5167 ,(1986) , 10.1016/S0021-9258(19)89228-1
D Najarian, M E Dihanich, N C Martin, A K Hopper, DNA sequence and transcript mapping of MOD5: features of the 5' region which suggest two translational starts. Molecular and Cellular Biology. ,vol. 7, pp. 185- 191 ,(1987) , 10.1128/MCB.7.1.185
K NASMYTH, K TATCHELL, The structure of transposable yeast mating type loci Cell. ,vol. 19, pp. 753- 764 ,(1980) , 10.1016/S0092-8674(80)80051-1
Gérard Faye, Frédéric Sor, Analysis of mitochondrial ribosomal proteins of Saccharomyces cerevisiae by two dimensional polyacrylamide gel electrophoresis. Molecular Genetics and Genomics. ,vol. 155, pp. 27- 34 ,(1977) , 10.1007/BF00268557
H Ito, Y Fukuda, K Murata, A Kimura, Transformation of intact yeast cells treated with alkali cations. Journal of Bacteriology. ,vol. 153, pp. 163- 168 ,(1983) , 10.1128/JB.153.1.163-168.1983