Expression in Escherichia coli of a Chemically Synthesized Gene for Biologically Active Bovine Acidic Fibroblast Growth Factor
作者： David L. Linemeyer , Linda J. Kelly , John G. Menke , Guillermo Gimenez-Gallego , Jerry DiSalvo
DOI: 10.1038/NBT0987-960
关键词: FGF10 、 Escherichia coli 、 Gene expression 、 Fibroblast growth factor 、 Biology 、 Molecular biology 、 Fibroblast growth factor receptor 4 、 Expression vector 、 Fibroblast growth factor receptor 3 、 3T3 cells 、 Biochemistry 、 Biotechnology 、 Molecular medicine 、 Applied Microbiology and Biotechnology 、 Bioengineering 、 Biomedical engineering
摘要: A gene encoding bovine acidic fibroblast growth factor has been chemically synthesized, cloned and expressed as a biologically active protein in Escherichia coli. The 440 base pair was assembled by enzymatic ligation of 16 oligonucleotides into pBR322–derived expression plasmid downstream the hybrid tac promoter. Expressed recombinant mitogen comigrated with native brain–derived detected Western blot immunological analysis. product synthetic purified to apparent homogeneity demonstrates mitogenic activity for Balb/c 3T3 cells presence heparin equivalent mitogen. These results demonstrate feasibility expressing large amounts functional bacteria provide system site–specific mutagenesis protein.
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sci-hub.se 参考文章(46)
K. A. Thomas, G. Gimenez-Gallego, J. DiSalvo, D. Linemeyer, L. Kelly, J. Menke, Structure, Homologies and Activities of Acidic Fibroblast Growth Factor Molecular Biology of the Arterial Wall. pp. 41- 43 ,(1987) , 10.1007/978-3-642-83118-8_13
Peter Böhlen, Frederick Esch, Andrew Baird, Kenneth L. Jones, Denis Gospodarowicz, Human brain fibroblast growth factor. Isolation and partial chemical characterization. FEBS Letters. ,vol. 185, pp. 177- 181 ,(1985) , 10.1016/0014-5793(85)80765-1
Patricia Ann D'Amore, Michael Klagsbrun, Roy Lobb, Joachim Sasse, Yuen Shing, Robert Sullivan, Jeffrey Jacobs, Purification and characterization of heparin-binding endothelial cell growth factors. Journal of Biological Chemistry. ,vol. 261, pp. 1924- 1928 ,(1986) , 10.1016/S0021-9258(17)36031-3
J S Huang, S S Huang, M D Kuo, Bovine brain-derived growth factor. Purification and characterization of its interaction with responsive cells. Journal of Biological Chemistry. ,vol. 261, pp. 11600- 11607 ,(1986) , 10.1016/S0021-9258(18)67285-0
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
S. Thornton, S. Mueller, E. Levine, Human endothelial cells: use of heparin in cloning and long-term serial cultivation Science. ,vol. 222, pp. 623- 625 ,(1983) , 10.1126/SCIENCE.6635659
P. Walicke, W. M. Cowan, N. Ueno, A. Baird, R. Guillemin, Fibroblast growth factor promotes survival of dissociated hippocampal neurons and enhances neurite extension. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 3012- 3016 ,(1986) , 10.1073/PNAS.83.9.3012
J. Abraham, A Mergia, J. Whang, A Tumolo, J Friedman, K. Hjerrild, D Gospodarowicz, J. Fiddes, Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor. Science. ,vol. 233, pp. 545- 548 ,(1986) , 10.1126/SCIENCE.2425435
T Maciag, T Mehlman, R Friesel, A. Schreiber, Heparin binds endothelial cell growth factor, the principal endothelial cell mitogen in bovine brain Science. ,vol. 225, pp. 932- 935 ,(1984) , 10.1126/SCIENCE.6382607
Roger Guillemin, Frederick Esch, Naoto Ueno, Andrew Baird, Fred Hill, Luc Denoroy, Nicholas Ling, Denis Gospodarowicz, Primary structure of bovine brain acidic fibroblast growth factor (FGF). Biochemical and Biophysical Research Communications. ,vol. 133, pp. 554- 562 ,(1985) , 10.1016/0006-291X(85)90942-8