In vitro evaluation of a new injectable calcium phosphate material

摘要: The purpose of this study was to develop an injectable bone substitute (IBS) for percutaneous orthopedic surgery. multiphasic material used composed a 2% aqueous solution methylhydroxypropylcellulose (MHPC) and biphasic calcium phosphate (BCP, 60% hydroxyapatite 40% beta-tricalcium phosphate) in which MHPC served as the carrier 80-200 microm BCP granules. best BCP/polymer ratio determined by rheological properties higher content material. Steam sterilization more effective than gamma irradiation maintaining stability mixture conserving its physiochemical mechanical properties. vitro biocompatibility composite checked direct-contact cytotoxicity cell-proliferation assays. A preliminary vivo test performed rabbit using intraosseous implantations femoral epiphysis. Histological analysis done after 1, 2, 4, 10 weeks. Bone ingrowth into IBS, close association with granules, observed 1 week increased regularly from surface inward at At same time, smaller granules (less 80 microns diameter) were degraded resorbed. This biomaterial proved suitable cavity filling. water solubility viscosity polymer allow cells recolonize, situ bonding mineral phase.