Usefulness of a genotypic resistance test using dried blood spot specimens in African HIV-infected children with virological failure according to the 2010-revised WHO criteria.
作者： Charlotte Charpentier , Jean-Chrysostome Gody , Pascaline Tisserand , Mathieu Matta , Julien Fournier
DOI: 10.1007/S00705-011-0997-9
关键词: Who criteria 、 Drug resistance 、 Virology 、 Population 、 Veterinary medicine 、 Biology 、 Dried blood spot 、 Genotype 、 Virological failure 、 Genotypic resistance 、 Concordance
摘要: We compared paired plasma and dried blood spot (DBS) samples from 54 HIV-1-treated children living in Bangui, Central African Republic, for antiretroviral-resistance-associated mutations. All displayed virological failure (HIV-1 RNA >3.70 log10copies/ml). Testing resistance genotype was carried out a reference laboratory Paris, France. A successful test result obtained (100%) plasmas 25 DBSs (46%). Among the 732 resistance-associated mutations analyzed, 718 were identical, leading to high concordance rate of 98.1%. Genotypic tests on DBS found be highly feasible accurate foreign laboratory, but with additional costs shipping decreased sensitivity.
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