High concordance between HIV-1 drug resistance genotypes generated from plasma and dried blood spots in antiretroviral-experienced patients
作者： Silvina Masciotra , Carolina Garrido , Ae S Youngpairoj , Amanda McNulty , Natalia Zahonero
DOI: 10.1097/QAD.0B013E3281C618DB
关键词: Polymerase chain reaction 、 Reverse transcriptase 、 Viral load 、 Nested polymerase chain reaction 、 Blood plasma 、 Virology 、 HIV drug resistance 、 Reverse-transcriptase inhibitor 、 Biology 、 Drug resistance
摘要: OBJECTIVE Dried blood spots (DBS) are a convenient alternative to plasma for drug resistance testing in resource-limited settings. We investigated the correlation between genotypes generated from DBS and plasma. DESIGN Sixty specimens HIV-1 subtype B-infected antiretroviral-experienced (n = 58) naive patients 2) were tested. prepared using 50 mul stored with desiccant at -20 degrees C. METHODS Resistance obtained ViroSeq assay compared derived The frequency of amplification proviral DNA was evaluated an in-house nested polymerase chain reaction assay. RESULTS : Fifty 60 successfully genotyped including all 38 collected viral loads greater than 2000 copies/ml 12 22 (54.5%) less copies/ml. detected 44.4% DBS. Despite presence DNA, highly concordant. Of 316 mutations found sequences, 306 (96.8%) also Discrepancies mostly caused by mixtures minor protease positions or unusual amino acid changes, only two cases major (M46L) reverse transcriptase (K103N) absent sequences. CONCLUSION demonstrated high concordance DBS, that can achieve sensitive levels similar those seen Our results indicate may represent feasible treated individuals.
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