Liver transcriptomic and plasma metabolomic profiles of fattening lambs are modified by feed restriction during the suckling period.

摘要: The increasing world population is driving demand for improved efficiency of feed resources livestock. However, the molecular mechanisms behind various traits and their regulation by nutrition remain poorly understood. Here, we aimed to identify differentially expressed (DE) genes in liver tissues fattening Merino lambs differences metabolites accumulated plasma modified metabolic pathways as a consequence milk restriction during suckling period. Twenty-four male (4.81 ± 0.256 kg) were divided into 2 groups (n = 12 per dietary treatment). first group (ad libitum, ADL) was kept permanently with dams, whereas other (restricted, RES) restricted. When they reached 15 kg live body weight (LBW), all animals offered same complete pelleted diet at level (35 g DM/kg LBW day) ensure no dry matter intake. All harvested when 27 LBW. For transcriptomic analysis, 4 samples from each (8 total) selected RNA sequencing (RNA-seq), (24 used perform nontargeted metabolomic analysis on hybrid quadrupole-time-of-flight mass spectrometer coupled an ultra-high performance liquid chromatographic system (UHPLC/QTOF-MS). Thirty-eight DE annotated identified RNA-seq, 23 being down-regulated up-regulated RES relative ADL (P < 0.10). Also, assay 38 compounds In general, those involved protein synthesis or protease inhibitors group, related proteolytic degradation up-regulated, thus suggesting higher catabolism proteins these lambs. showed over-expression xenobiotic metabolism pathways, β-oxidation fatty acids down-regulated. According data obtained, early period promoted long-term effects both hepatic profile profile, which might have metabolism, proteins, detoxification xenobiotics, reducing