Identification of an adenosine 3',5'-monophosphate (cAMP)-responsive region in the rat growth hormone gene: evidence for independent and synergistic effects of cAMP and thyroid hormone on gene expression.
作者： Richard P. Copp , Herbert H. Samuels
DOI: 10.1210/MEND-3-5-790
关键词: Activator (genetics) 、 Gene expression 、 PDE10A 、 Growth-hormone-releasing hormone receptor 、 Biology 、 Internal medicine 、 Thyroid hormone receptor 、 Hormone 、 CREB1 、 Endocrinology 、 Forskolin
摘要: Rat GH gene expression is known to be stimulated by several factors, including thyroid hormone and GRF. This effect of GRF appears mediated cAMP resulting from activation adenylate cyclase the peptide. The elements rat important for stimulation cell-specific have been previously mapped using transfection techniques. Cell-specific two located −137 −107 −95 −65. Sequences mediating are thought between −208 −160. In this study, three different methods elevate levels in cells [forskolin, a direct activator catalytic subunit; 8-(4-chlorophenylthio)cAMP, nonmetabolizable analog; isobutylmethylxanthine, phosphodiesterase inhibitor], we show that 5′-flanking DNA can mediate (10- 20-fold). cAMP-responsive region was se...
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