The effect of hydrogen peroxide upon β-adrenoceptor density and function in C6 rat glioma cells
作者： D Bergström , SO Jacobsson , CJ Fowler
DOI: 10.1016/S0197-0186(98)00070-9
关键词: Beta-1 adrenergic receptor 、 Ligand (biochemistry) 、 Adenylyl cyclase 、 Hydrogen peroxide 、 Cell culture 、 Biophysics 、 Stimulation 、 Chemistry 、 Biochemistry 、 Cell 、 Forskolin
摘要: Hydrogen peroxide has been suggested to play an important role in the pathogenesis of Alzheimer's disease. In present study, effects hydrogen upon functional integrity beta-adrenoceptors have investigated C6 glioma cells. Treatment cells for 24 h with serum-free medium produced a concentration-dependent cell toxicity, seen both using counting and LDH release into as end point. There were no large nor consistent changes either density surface beta 1, or 2-adrenoceptors, measured hydrophilic ligand [3H](-)-CGP 12177, basal, forskolin isoprenaline-stimulated cAMP responses, following treatment. It is concluded that decreased adenylyl cyclase activity responsiveness Gs stimulation found post-mortem brain samples from disease autopsy cases unlikely be mediated by peroxide.
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