The impact of feeding β2-adrenergic agonists on rainbow trout muscle β2-adrenoceptors and protein synthesis
作者： M. B. Lortie , T. Arnason , S. G. Dugan , J. G. Nickerson , T. W. Moon
DOI: 10.1111/J.0022-1112.2004.00482.X
关键词: Clenbuterol 、 Biology 、 Sarcoplasm 、 Ractopamine 、 Adrenergic receptor 、 Endocrinology 、 Complete protein 、 Myofibril 、 Internal medicine 、 Skeletal muscle 、 Rainbow trout
摘要: The β2-adrenergic agonists (β 2 -AAs) clenbuterol (CLEN) and ractopamine (RACT) were fed to rainbow trout Oncorhynchus mykiss for 30-37 days red white muscle β -adrenoceptor -AR) binding characteristics mRNA expression assessed in parallel with fractional protein synthetic rates. Feeding CLEN or RACT had no significant effect on any body physiological variables measured. There differences the number of sites (Borax) while a increase K d was observed β-ARs membranes isolated from -AA-fed trout. No change -AR levels -AA feeding, implying that these -AAs do not act at transcriptional level least -AR. treatments, however, did significantly synthesis rates whole protein, myofibrillar sarcoplasmic soluble fractions. Although conclusive, this study supports direct link between -ARs synthesis.
sci-hub.se 参考文章(46)
H J Mersmann, Overview of the effects of beta-adrenergic receptor agonists on animal growth including mechanisms of action Journal of Animal Science. ,vol. 76, pp. 160- 172 ,(1998) , 10.2527/1998.761160X
Peter F. Dubbelhuis, Alfred J. Meijer, Chapter 14 – Amino Acid-dependent Signal Transduction Cell and Molecular Response to Stress. ,vol. 3, pp. 207- 219 ,(2002) , 10.1016/S1568-1254(02)80016-X
D. E. Moody, D. L. Hancock, D. B. Anderson, Phenethanolamine repartitioning agents. Farm animal metabolism and nutrition.. pp. 65- 96 ,(2000) , 10.1079/9780851993782.0065
H. H. Meyer, L. M. Rinke, The pharmacokinetics and residues of clenbuterol in veal calves. Journal of Animal Science. ,vol. 69, pp. 4538- 4544 ,(1991) , 10.2527/1991.69114538X
M. E. Spurlock, J. C. Cusumano, S. Q. Ji, D. B. Anderson, C. K. Smith, D. L. Hancock, S. E. Mills, The effect of ractopamine on beta-adrenoceptor density and affinity in porcine adipose and skeletal muscle tissue. Journal of Animal Science. ,vol. 72, pp. 75- 80 ,(1994) , 10.2527/1994.72175X
G L Waldo, J K Northup, J P Perkins, T K Harden, Characterization of an altered membrane form of the beta-adrenergic receptor produced during agonist-induced desensitization. Journal of Biological Chemistry. ,vol. 258, pp. 13900- 13908 ,(1983) , 10.1016/S0021-9258(17)44003-8
P D Warriss, S C Kestin, T P Rolph, S N Brown, The effects of the beta-adrenergic agonist salbutamol on meat quality in pigs. Journal of Animal Science. ,vol. 68, pp. 128- 136 ,(1990) , 10.2527/1990.681128X
James G. Nickerson, Stephen G. Dugan, Guy Drouin, Thomas W. Moon, A putative β2
-adrenoceptor from the rainbow trout (Oncorhynuchus mykiss
) European Journal of Biochemistry. ,vol. 268, pp. 6465- 6472 ,(2001) , 10.1046/J.0014-2956.2001.02600.X
P. Rajab, J. Fox, S. Riaz, D. Tomlinson, D. Ball, P. L. Greenhaff, Skeletal muscle myosin heavy chain isoforms and energy metabolism after clenbuterol treatment in the rat American Journal of Physiology-regulatory Integrative and Comparative Physiology. ,vol. 279, ,(2000) , 10.1152/AJPREGU.2000.279.3.R1076
P J Garlick, M A McNurlan, V R Preedy, A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine Biochemical Journal. ,vol. 192, pp. 719- 723 ,(1980) , 10.1042/BJ1920719