Studies on hyaluronic acid: I. The preparation and properties of rooster comb hyaluronic acid

摘要: Abstract 1. Hyaluronic acid has been prepared quantitatively from the rooster comb under mild conditions, with a yield of 6% acetone-dried combs. 93% this hyaluronic was extracted distilled water, while stronger treatments, 1 M NaCl extraction and digestion pronase, were required to remove remainder tissues. 2. The crude extracts purified by modified Sevag 's procedure, epichlorohydrin triethanolamine- (ECTEOLA-) chromatography fractionation cetylpyridinium chloride. these procedures recovered greater than 90% respect hexuronic acid. 3. Analysis products physical chemical methods showed that acids in each fraction identical their glucosamine content, infrared absorption, optical rotation electrophoretic mobility. Viscosity measurements molecular weight determinations using sedimentation equilibrium method be different sizes. 4. significance results is discussed relation known structure composition comb.