Contribution of bacterial detachment by enzymatic or physical methods to the diagnosis of foreign material infections and chronic osteomyelitis

摘要: Bacterial adhesion is a crucial factor in the pathogenicity of numerous bacterial species. This property plays such great role infections foreign material that removal only effective therapy. The electron microscopy studies performed by Christensen have demonstrated infected harbors bacteria which are often coated with slime and therefore escape detection conventional microbiological methods. view these properties, enzymatic or physical detachment adherent was attempted. 231 specimens from variety materials (catheters, heart valves, drains, bone biopsies) yielded negative results after 15 days culture Schaedler medium were either exposed to trypsin sonicated. Among catheter specimens, 44.7% positive bacteriologic results; pathogens recovered included 8.3% micrococci, 5.1% nongroupable streptococci, 1.7% group D 2.9% Enterobacteria, 23.3% coagulase-negative staphylococci. improvement yield 64.7%; except one case, consistent previously initiated treatments. method should be studied larger number determine its diagnostic value as compared techniques.