Human hematopoietic CD34+ progenitor cells induce natural killer cell alloresponses via NKG2D activation.

摘要: Human CD34+ cells cross-interact with allogeneic T lymphocytes. In this study we addressed the interaction between and natural killer (NK) cells. Purified NK were cultured KIR-permissive or CD14+ blood cells, obtained from HLA group C homozygous donors, high-dose interleukin-2. A cytotoxicity assay was used to test ability of lyse NK-sensitive K562 NK-resistant Daudi Cytofluorometric assays employed assess cell phenotype cytokine release. induced greater lysis ( p  = 0.02)  = 0.01) than monocytes. CD34 stimulation resulted in upregulation CD69 CD25 on production interferon γ tumor necrosis factor α. activation by inhibited an anti-NKG2D antibody. However, NKG2D ligands such as MIC (MHC class I chain)-A/B ULBP (UL16 binding protein)-1/3 not detected CD34+ cells. Cross-talk also CD40 CD86 co-stimulatory molecules Our indicates a direct NKG2D-dependent stimulatory effect human These findings may be relevant NK-mediated rejection HLA-mismatched hematopoietic stem transplantation.